Microbial fertilizer is also known as bacterial fertilizer, popularly speaking is bacteria + fertilizer, which is a bio-active microbial fertilizer that promotes the growth of crops with microbial life activities and their products, and promotes the absorption of nutrients by plants through microbial life activities, so that plants can obtain specific fertilizer effects. Reasonable application of bacterial manure can not only improve the soil, but also help to improve the resistance of crops to biotic and abiotic stresses, improve the yield and quality of crops, and provide more possibilities for the development of organic agriculture.
Why use Corn Steep Liquor and B. subtilis to make microbial fertilizer?
Corn Steep Liquor is rich in soluble protein, amino acids, vitamins, growth factors, various minerals and other nutrients, and its high ammonia nitrogen content is conducive to the increase of biomass, and is widely used in high-density fermentation of functional microorganisms. It has great application potential and broad development prospect in agricultural field.
B. subtilis has many advantages, such as salt tolerance, low temperature and high temperature resistance, secretion of high-quality protease, etc. It has also been proved to promote plant growth, It is a beneficial bacterium widely used in microbial fertilizers. The protease produced by B. subtilis can decompose macro-molecular proteins in corn steep liquor into amino acids, increase the content of free amino acids in fertilizer, and facilitate the absorption and utilization of crops.
The number of viable bacteria is the key index to evaluate the quality of microbial inoculants. In the study, the number of viable bacteria of B. subtilis produced in corn steep liquor medium was significantly higher than that in peptone medium. corn steep liquor is a high-quality and inexpensive nitrogen source, the price is much lower than peptone, yeast extract, etc., can reduce the cost of B. subtilis culture medium, and then reduce the production cost of microbial fertilizer;
Precautions for making bacterial fertilizer by corn steep liquor fermentation
1: The PH range suitable for the growth of B. subtilis is neutral and acidic. During fermentation, the consumption of amino acid substrate causes the PH to rise, so a neutralizer needs to be added after the PH exceeds 7 to adjust.
2: In the process of corn steep liquor fermentation, the amount of inoculation, the interaction of corn steep liquor concentration and pH have significant effects on the results, and small-scale experiments should be conducted to determine the most suitable fermentation parameters before large-scale application.
3: The fermentation process should pay attention to the content of soluble oxygen, the decrease of oxygen content will limit the high-density fermentation of B. subtilis;
4: Using corn steep liquor as medium to ferment B. subtilis to produce microbial fertilizer; The number of viable bacteria and biomass of B. subtilis were significantly increased by adding glucose during the process.
